Connexin 43, Mouse (Cx43) aa252-270 (Cx43, Gap Junction Alpha 1 Protein, C

Control Peptide for C7857 (monoclonal antibody) and C7857-02 (polyclonal antibody).\n\nMouse connexin 43 is a 382 amino acid gap junction protein with a predicted MW ~43kD. It is prominently expressed in heart. Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1kD. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Absence of intercellular communication mediated by gap junction may lead to transformed or cancerous growth. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane domains: 2 extracellular (EC) and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic.\n\nApplications:\nSuitable for use in ELISA. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7857-04

Size

100ug

Applications

ELISA

Reactivities

Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives added.

Purity

Highly purified

References

1. Kumar, N. and Giula, N. (1996) Cell 84: 381-388. 2.

Additional Info

Synthetic peptide corresponds to a 19aa sequence (aa252-270) within the C-terminal cytoplasmic domain of Mouse Connexin 43. Species sequence homology: Rat and bovine 100%.