Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3)

Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific. They allow passive diffusion of small molecules up to 1000D. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junctions play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane (TM), 2 extracellular (EC) and 3 cytoplasmic (CP) domains. The EC, TM and N-terminal CP domains are well conserved among family members. The Central and C-terminal domains are highly variable in both sequence and size. The N- and C-termini are predicted to be cytoplasmic.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml; Using ECL. The protein detected may migrate slightly faster than the 46kD.\nELISA: 1:100,000. Using 50-100ng C7858-07B Control Peptide/well.\nImmunohistochemistry: 2-10ug/ml. Adherent cells can be fixed in 50% methanol-50% acetone or 1% paraformaldhyde. \nOptimal dilutions to be determined by the researcher\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7858-07A

Size

50ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

US Biological application reference: Banerjee, D. et al., (2010) Intl. J. Cancer 127; 839-848. 1. Fischer, R. (2005) Gastroenterology, 128; 433-448. 2. Laux-Fenton, W. (2003) Cornea. 22:457-464. 3. Jacobs, M.D., (2004), Invest. Ophthalmol.Vis.Sci, 45: 191-199. 4. Kumar, N.M. and Giula, N.B. (1996) Cell 84, 381-388; 5. White , W.T. et al (1995) Kidney Intl. 48, 1148-1157; 6. Evans, HW (1994) Biochem. Soc. Tr. 788-792; 7. Byer, E et al (1990) J. membrane Biol. 116, 187-194 8. Paul DL et al (1991) J Cell Biol. 115, 1077-1089. 9.. Zampighi, G.A. et al., (2005) Invest. Ophthalmol. Vis. Sci., 46: 3247. 10. Isakson, B.E. et al., (2001) Am J Physiol Cell Physiol 281: C1291. 11. Merriman-Smith, B.R.et al., (2003) Invest. Ophthalmol. Vis. Sci., 44: 3458 - 3466. 12. Yoshida, M. et al., (2005) Pathology Intl 55; 732-737.

Additional Info

Recognizes rat Cx46. No significant homology is seen with other Connexins.