Connexin 47

Intercellular communication through gap junctions (connexins) plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation and growth control. In rodents, 15 connexin genes have been described. Specific cell types in the brain express specific types of connexins; expression patterns coincide with tissue compartmentalization and function. These compartments change during development5. Reduction or alteration in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis. The coding sequence of the mouse gap junction Cx47 has 49% homology with mouse Cx45; both are located on chromosome 11 in the mouse genome. Cx47 has been observed to express mainly in the gray matter of the central nervous system, specifically in several types of hippocampal, cortical, cerebellar and spinal cord neurons. Functional gap junctions are involved in tissue homeostasis of ions, metabolites and second messenger molecules. Between neuronal cells, these functions play a role in synchronizing oscillations of certain cell clusters and could impact burst thresholds of electrotonically coupled cells. Cx47 induces strong electrical coupling that is sensitive to chemical uncouplers. Cx47 appears to be a less restrictive channel than Cx36 and may show preference to larger metabolites or second messengers rather than the intercellular diffusion of inorganic ions that Cx36 preferentially allows.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-3ug/ml\nImmunohistochemistry (Frozen): 2-5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7859

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bennet MV, et al. Neuron 6(3): 305-320, 1991. 2. Kumar NM & Gilula NB. Cell 84(3): 381-388, 1996. 3. Kuraoka A. J Histochem Cytochem 41(7): 971-980, 1993. 4. Manthey D, et al. J Biol Chem 274(21): 14716-14723, 1999. 5. Rozental R, et al. Brain Res Brain Res Rev 32(1): 11-15, 2000. 6. Schwartz HJ, et al. Somat Cell Mol Genet 18(4): 351-359, 1992. 7. Sutor B, et al. Cereb Cortex 10(7): 684-697, 2000. 8. Teubner B, et al. J Neurosci 21(4):1117-1126, 2001. 9. White TW, et al. Annu Rev Physiol 61: 283-310, 1999. 10. Wilgenbus KK, et al. Int J Cancer 51(4): 522-529, 1992. 11. Willecke K, et al. Novartis Found Symp 219: 76-88, 1999.

Additional Info

Recognizes the mouse Connexin 47 protein. On Western Blots of mouse brain homogenates, it identi?ed a single band at ~47kD. Reactivity has been con?rmed in Western Blots with mouse thalamus, spinal cord, hypothalamus, cerebellum and whole brain lysates.