Connexin 47\n

Intercellular communication through gap junctions (connexins) plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation and growth control.1-3 In rodents, 15 connexin genes have been described4,9. Specific cell types in the brain express specific types of connexins; expression patterns coincide with tissue compartmentalization and function. These compartments change during development5. Reduction or alteration in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis10. The coding sequence of the mouse gap junction Cx47 has 49% homology with mouse Cx45; both are located on chromosome 11 in the mouse genome6,8. Cx47 has been observed to express mainly in the gray matter of the central nervous system, specifically in several types of hippocampal, cortical, cerebellar and spinal cord neurons8. Functional gap junctions are involved in tissue homeostasis of ions, metabolites and second messenger molecules10. Between neuronal cells, these functions play a role in synchronizing oscillations of certain cell clusters and could impact\nburst thresholds of electrotonically coupled cells7. Cx47 induces strong electrical coupling that is sensitive to chemical uncouplers8. Cx47 appears to be a less restrictive channel than Cx36 and may show preference to larger metabolites or second messengers rather than the intercellular diffusion of inorganic ions that Cx36 preferentially allows8.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1-3ug/ml\nWestern Blotting: 1-3ug/ml\nImmunoprecipitation: 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\n

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SPECIFICATIONS

Catalog Number

C7859-01

Size

100ug

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Highly purified from mouse ascites by protein A chromatography.

Isotype

IgG1,k

References

1. Bennet MV, et al. Neuron 6(3): 305-320, 1991.\n2. Kumar NM & Gilula NB. Cell 84(3): 381-388, 1996.\n3. Kuraoka A. J Histochem Cytochem 41(7): 971-980, 1993.\n4. Manthey D, et al. J Biol Chem 274(21): 14716-14723, 1999.\n5. Rozental R, et al. Brain Res Brain Res Rev 32(1): 11-15, 2000.\n6. Schwartz HJ, et al. Somat Cell Mol Genet 18(4): 351-359, 1992.\n7. Sutor B, et al. Cereb Cortex 10(7): 684-697, 2000.\n8. Teubner B, et al. J Neurosci 21(4):1117-1126, 2001.\n9. White TW, et al. Annu Rev Physiol 61: 283-310, 1999.\n10. Wilgenbus KK, et al. Int J Cancer 51(4): 522-529, 1992.\n11. Willecke K, et al. Novartis Found Symp 219: 76-88, 1999.

Additional Info

Reacts with the mouse Connexin 47 protein. On Western blots of mouse thalamus homogenates and Connexin 47-transfected HeLa cells, it identified a band at ~47kD. Reactivity was confirmed in Western blots with mouse thalamus lysates. Mouse Connexin 47-transfected HeLa cells were used in both Immunoprecipitation and Western blot assays. This antibody was also used in immunofluorescence assays using paraformaldehyde-fixed mouse brain. The expected staining pattern is punctate labeling of the oligodendrocytes in the brain.