Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150kD member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. Its exact function is unclear, but it likely mediates cell-cell interaction and may promote
neurite outgrowth. Human Contactin-3 preproprecursor is 1028aa in length. It contains a 19aa signal sequence, a 983aa mature region and a 26aa cleavable C-terminal prosegment. The mature region shows six C2- type IG-like domains (aa26-593), followed by four fibronectin type III modules (aa598- 992). There is one potential alternate start site at Met128. Over aa26-992, human Contactin-3 shares 92% aa identity with mouse Contactin-3.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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