Contactin-associated protein 1 (Caspr1)

Contactin-associated protein 1(Caspr) is a single-pass type I membrane protein belonging to the neurexin family and is comprised of one F5/8 type C domain, one fibrinogen C-terminal domain, two EGF-like domains, and four laminin G-like domains. Caspr is well documented for its interaction with contactin, an interaction that is crucial to the transport of Caspr to the plasma membrane, the mylination of glial cells, and the generation of axoglial junctions. Studies also suggest that Caspr may play a regulatory role in the cell surface expression of contactin and its targeting to different axonal domains. Expression of Caspr is largely observed in the brain, mainly in CNS myelinated nerve fibers within paranodal axoglial junctions.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemistry: 1:300 detects Caspr in rat cerebellum and rat hippocampus tissues.\nWestern Blot: 0.5ug/ml detected Caspr on 10ug of rat brain membrane tissue lysate.\nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nRat brain membrane tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7865-19A

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Wolswijk, G., et al. (2003). Brain. 126:1638- 1649. 2. Rios, J., et al. (2000). J. Neurosci. 20:8354. 3. Gollan, L., et al. (2003). J. Cell. Biol. 163:1213.

Additional Info

Recognizes rat Caspr. Species sequence homology: mouse 100%.

Alternative Names

contactin associated protein 1, neurexin 4, neurexin-4\ncontactin-associated protein 1, Neurexin IV, Neurexin-4