COP, beta (Coatomer Protein beta)

Applications: \nSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunocytochemistry and AF. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7900-50A1

Size

100ul

Applications

ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, NHP

Form

As reported

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

Kreis TE et al. COPs regulating membrane traffic. Annu Rev Cell Dev Biol 11:677-706 (1995). <PUBMED:8689572>Griffiths G et al. Immunocytochemical localization of beta-COP to the ER-Golgi boundary and the TGN. J Cell Sci 108 (Pt 8):2839-56 (1995). <PUBMED:7593324>Whitney JA et al. Cytoplasmic coat proteins involved in endosome function. Cell 83:703-13 (1995). <PUBMED:8521487>Pepperkok R et al. Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo. Cell 74:71-82 (1993). <PUBMED:8334707>Oprins A et al. Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas. J Cell Biol 121:49-59 (1993). <PUBMED:8458872>Narula N et al. Identification of a 200-kD, brefeldin-sensitive protein on Golgi membranes. J Cell Biol 117:27-38 (1992). <PUBMED:1556155>Duden R et al. Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin. Cell 64:649-65 (1991). <PUBMED:1840503>Ktistakis NT et al. PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A. J Cell Biol 113:1009-23 (1991). <PUBMED:1710224>Donaldson JG et al. Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action. J Cell Biol 111:2295-306 (1990). <PUBMED:2277061>Allan VJ & Kreis TE A microtubule-binding protein associated with membranes of the Golgi apparatus. J Cell Biol 103:2229-39 (1986). <PUBMED:3536963>

Additional Info

Recognizes an epitope in the beta-COP protein (110kD). The antibody stains the periphery of the Golgi complex in immunohistology using cultured cells. Species Crossreactivity: Crossreacts with Hamster, Human, Rat and Monkey.

Alternative Names

Beta-coat protein, betacop, Coatomer beta subunit, Coatomer protein complex subunit beta 1, Coatomer protein complex subunit beta, COPB1, DKFZp761K102, FLJ10341