COP9 Signalosome Subunit 5 (COPS5, Jun Activation Domain-binding Protein 1, JAB1, MGC3149, MOV-34, SGN5)

Jun activation domain-binding protein 1 (JAB1, also known as CSN5) is a co-activator of AP-1 that was first identified in its interaction with the activation domain of c-Jun. JAB1 is also a component of the CSN multiprotein complex, which modulates signal transduction, gene transcription, and protein stability. JAB1 interacts with p27, shuttling it from the nucleus to the cytoplasm and decreasing the level of p27 in the cell by increasing its degradation via the ubiquitin-proteasome pathway. An inverse relationship in expression levels of p27 and JAB1 has been reported in various cancer tissues. In a study of 80 cases of epithelial ovarian tumors, JAB1 expression correlated inversely with p27 expression, with JAB1 overexpression significantly associated with poor patient prognosis. Immunohistochemical analysis of a series of 53 invasive breast carcinoma specimens and their adjacent normal breast tissue counterparts also demonstrated an inverse association between JAB1 and p27; patients with no JAB1 expression had the highest survival rate, while patients with high JAB1 levels had the lowest survival rate. JAB1 and p27 are also inversely related in anaplastic large cell lymphoma (ALCL); however, in all 54 patients examined, p27 expression correlated with worse overall survival in both ALK-positive and ALK-negative groups.\n\nApplications:\nSuitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoprecipitation: 1-3ug/ml\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

C7900-58B

Size

100ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG1,k

References

1. Claret FX, et al. Nature 383(6599):453-457, 1996.2. Tomoda K, et al. Nature 398:160-165, 1999.3. Tomoda K, et al. J Biol Chem 277(3):2302-2310, 2002.4. Sui L, et al. Clin Cancer Res 7(12):4130-4135, 2001.5. Kouvaraki MA, et al. Cancer Res 63:2977-2981, 2003.6. Rassidakis GZ, et al. Clin Cancer Res 9:1121-1128, 2003.

Additional Info

Recognizes human JAB1 protein at ~40kD. Reactivity has been confirmed with human 293T and Jurkat cell lysates by Western Blot and immunoprecipitation.