Copper Assay Kit, BioAssay(TM)

Copper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin and collagen. Copper is mainly present in caeruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anaemia, hypotonia and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson's disease, microcytic hypochromic anaemia and bone disease due to reduced collagen synthesis.\n\nSimple, direct and automation-ready procedures for measuring copper concentrations find wide applications in research, drug discovery and environmental monitoring. Copper assay kit is designed to measure copper with no or minimal sample treatment. The improved method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured at 359nm, is directly proportional to copper concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.\n\nKey Features:\nSensitive and accurate. Linear detection range 7 ug/dL (1.0uM) to 300ug/dL (47uM) copper in 96-well plate assay.\nSimple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.\nImproved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.\n\nApplications:\nDirect Assays: biological, environmental, food and beverage samples.\nDrug Discovery/Pharmacology: effects of drugs on Cu metabolism.\n\nKit Contents: (250 tests in 96-well plates)\nReagent A: 10ml Reagent B: 1.5ml Reagent C: 40ml\nCopper Standard: 1ml 1.5 mg/dL Cu2+\nStorage conditions. The kit is shipped at room temperature. Store all reagents at 4 degrees C. Shelf life: 12 months after receipt. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.\n\nProcedures:\nNote: metal chelators (e.g. EDTA) interfere with this assay and should be avoided in sample preparation.\n\nProcedure using 96-well plate:\n1. Standards: transfer 100ul dH2O into one Eppendorf tube labeled

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SPECIFICATIONS

Catalog Number

C7900-59K

Size

1Kit

References

1. Stuerenburg HJ, Eggers C (2000). Early detection of non- compliance in Wilson's disease by consecutive copper determination in cerebrospinal fluid. J Neurol Neurosurg Psychiatry 69: 701-702.\n2. Liska SK, Kerkay J, Pearson KH (1985). Determination of zinc and copper in urine using Zeeman effect flame atomic absorption spectroscopy. Clin Chim Acta. 151: 231-236.\n3. Tessman RK, Lakritz J, Tyler JW, Casteel SW, Williams JE, Dew RK. (2001). Sensitivity and specificity of serum copper determination for detection of copper deficiency in feeder calves. J Am Vet Med Assoc. 218: 756-760.