Copper Transporting ATPase1 (ATP7A, ATPase Cu transporting, Copper pump 1, Menke)

The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-trans locating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\n1ug/ml was sufficient for detection of Copper-transporting ATPase1 in 20ug of rat brain lysate by Western Blot using Goat IgG:HRP as the secondary antibody.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

167581

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS pH7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Samimi G., et al. (2003) Clin. Cancer Res. 9: 5853-9. 2. Samimi G., et al. (2004) Mol Pharmacol. 66: 25-32.\n3. Greenough M., et al. (2004) Am. J. Physiol. Cell Physiol. 287: C1463-71.\n4. Song, I.S., et al. (2004) Mol. Cancer Ther. 3: 1543-1549.\n5. van Dongen, E.M., et al. (2004) Biochem. Biophys. Res.\nCommun. 323: 789-795.\n6. Samimi, G., et al. (2004) Mol Pharmacol 66: 25-32.\n7. Morgan, C.T., et al. (2004) J. Biol. Chem. 279: 36363-36371.\n8. Barnes, N., et al. (2005) J. Biol. Chem. [Epub].\n

Additional Info

Recognizes human Copper Transporting ATPase1. Species Crossreactivity: Human, Mouse, Rat. Detects ~180kD in rat brain membrane preparations