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COPS5 (COP9 Signalosome Complex Subunit 5, SGN5, Signalosome Subunit 5, Jun Activation Domain-binding Protein 1, CSN5, JAB1)

Cat no: C7900-58L

COPS5 (COP9 Signalosome Complex Subunit 5, SGN5, Signalosome Subunit 5, Jun Activation Domain-binding Protein 1, CSN5, JAB1)

Jun activation domain-binding protein-1 (JAB1) also designated as COP9 subunits 5 (COPS5) or SGN5, is a co-activator of AP-1 transcription factor that promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP-1 containing complexes, and enhances transactivation from AP-1-dependent promoters. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized in both the nucleus and the cytoplasm.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml for 2h at RT\nImmunohistochemistry: 1:200 for 30min at RT\nStaining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBreast carcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7900-58L

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human JAB1. Species Crossreactivity: mouse and rat.

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