Core Binding Factor alpha 3 (CBFA3)

The gene noggin encodes a member of one of at least four distinct gene families encoding secreted polypeptides that bind tomembers of the transforming growth factor-beta superfamily, suchas BMP, and inhibit the function of these signaling proteinsby preventing their interaction with receptors on the cell surface. Other antagonists with related functions includeChordin, Follistatin, Sclerostin and members of the DAN family. These BMP antagonists are assumed to be diffusibleand therefore potentially important in the establishment of BMPactivity gradients in vivo Although structurally distinct, members of these gene familieshave in some cases similar ligand specificity and overlappingpatterns of expression, and in the case of Chordin and Nogginthese proteins apparently are capable of at least partial compensationfor each other. In addition to these multiple secreted BMP antagonists, there are other secreted proteins whose primaryfunction is to overcome this antagonism. Thus, thereis a highly complex system to regulate the bioavailability andconsequently the activities of BMPs in the extracellularspace.\n\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1-10ug/ml (ECL).\nELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.\n\nControl Peptide:\nC7900-74: Core Binding Factor alpha 3 (CBFA3)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C7900-74

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. M. C. Ghozi et al (1996) PNAS, Vol. 93, 1935-40; D. Levanon et al (1994). 2. Genomics 23, 425-432; strausberg, R. L et al (2002) PNAS. 99 (26), 16899-16903;. 3. D. Levanon et al (1998) PNAS 95 (20), 11590-11595.

Additional Info

Recognizes human CBFA3. Species Sequence Homology: mouse, rat - 92%.