Core Binding Factor alpha subunit 1 (CBFA1)

Core biding factors (CBF) are a family of heterodimeric transcription factors comprised of two subunits: A DNA binding a subunit and non-DNA binding b subunit. CBF alpha is encoded by three distinct genes (CBFA1/PEBP2aA/AML3, CBFA2/PEBP2aB/AML1, and CBFA3/PBEP2aC/AML2), and a b subunit encoded by CBFB/PEBP2b. The CBFa subunits share a conserved 128aa runt domain that is homologous to the Drosophila pair-rule gene runt that plays a role in the formation of the segmented body pattern, sex determination and development of the nervous system. The consensus DNA-binding sequence is PuACCPuCA, originally identified as an enhancer in polyoma virus and murine leukemia virus. The null mutation of cbfa2 and cbfb in mice were embryonic lethal. A disruption in cbfa1 gene product blocked osteoclast development from mesenchyme with no ossification. Mutations or deletion in cbfa1 have been shown to be associated with CCD, a human skeletal disorder named cleidocranial dysplasia. Cbfa1 is (alternatively spliced form 507 and 522aa in human and 528aa or 59aa in mouse) is expressed in bone, thymus and testis but not in brain, lung, heart, spleen, liver or kidney. Cbfa1 is an essential transcription factor in osteoblast differentiation.\n \nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 (ECL)\nELISA: 1:10,000-1:100,000. Coat with 1ug of control peptide per well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nC7900-72: Core Binding Factor alpha subunit 1, Human, Control Peptide (CBFA1)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7900-70

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid, 0.1% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Mundlos, S., et al., Cell 89: 773-779 (1997). 2. Ducy, P., et al., Cell 89: 747-754 (1997). 3. Komori, T., et al., Cell 89: 755-764 (1997). 4. Otto, F., et al., Cell 89: 765-771 (1997). 5. Rodan, G.A., Harada, S.-I., et al., Cell 89: 677-680 (1997).

Additional Info

Recognizes human Core Binding Factor alpha subunit 1 (CBFA1). No significant homology is seen with CBFA2/PEBP2aB/AML1 and CBFA3/PBEP2aC/AML2 or any runt family of proteins. Species Sequence Homology: mouse cbfa1/osf2: 100%.