CoREST (REST corepressor 1, Protein CoREST, KIAA0071, RCOR, RCOR1)

Applications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.5ug/ml detects CoREST in RIPA lysates from A431, 3T3/A31, Jurkat, and Hela cells. Reported to be detected in RIPA lysates from HEK 293, L6, PC12, C6 cells and liver, muscle, heart and brain tissue.\nImmunoprecipitation: 2.5ug immunoprecipitates CoREST from 250ug of A431 RIPA lysate. \nImmunocytochemistry: Positive immunostaining for CoREST in cells fixed with 4% paraformaldehyde pH 7.3 for 10-15 minutes\nImmunohistochemistry: Reported to detect CoREST in tissue sections. \n\nIncluded Positive Control: \nA0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7900-80

Size

200ug

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Lunyak, V. V., et. al., Science 298: 1747-1752, 2003. 2. Ballas, N., et. al., Neuron 31: 353-365, 2001. 3. Andres, M. E., et. al., PNAS 96: 9873-9878, 1999.

Additional Info

Recognizes human CoREST, Mr ~66kD. Species Crossreactivity: Mouse and rat.