Corneodesmosin, Recombinant, Human (CDSN, D6S586E, HTSS, S protein)

Corneodesmosin, al so known as CDSN and the S gene product , is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin. It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes. It is also expressed in the inner root sheath of hair follicles. Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains. Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes. Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness. Corneodesmosin is secreted by keratinocytes as a 52-56kD molecule which is then subjected to repeated sequential N- and C-terminal proteolysis. Species of 46, 43, 36, and 15kD are present in corneocytes. Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin's ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin. Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention. Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp.\n\nSource:\nMurine myeloma cell line, NS0-derived. Lys33-Pro528, with a C-terminal 6-His tag (Asp526Asn).\n\nMolecular Weight: 60kD (SDA-PAGE, reducing conditions) \n\nBiological Activity:\nMeasured by the ability of the immobilized protein to support the adhesion of BUD-8 human skin fibroblast cells. When 3x10e4 cells/well are added to CDSN coated plates (10ug/ml, 100ul/well), ~50-80% will adhere after 1h at 37 degrees C. \n\nEndotoxin: (same/less than)1EU/ug (LAL) \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with 200ug/ml PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7900-87

Size

50ug

Form

Supplied as a lyophilized powder from PBS. Carrier free. Reconstitute with 200ug/ml PBS.

Purity

(same/more than)95% (SDS-PAGE)

References

1. Pierard, G.E. et al. (2000) Int. J. Mol. Med. 6:217. 2. Zhou, Y. and D.D. Chaplin (1993) Proc. Natl. Acad. Sci. 90:9470. 3. Haftek, M. et al. (1997) Br. J. Dermatol. 137:864. 4. Simon, M. et al. (1997) J. Biol. Chem. 272:31770. 5. Mils, V. et al. (1992) J. Histochem. Cytochem. 40:1329. 6. Matsumoto, M. et al. (2008) Proc. Natl. Acad. Sci. 105:6720. 7. Guerrin, M. et al. (1998) J. Biol. Chem. 273:22640. 8. Jonca, N. et al. (2002) J. Biol. Chem. 277:5024. 9. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:747. 10. Leclerc, E. A. et al. (2009) J. Cel l Sci . 122:2699. 11. Simon, M. et al. (2001) J. Biol. Chem. 276:20292. 12. Simon, M. et al. (2008) Br. J. Dermatol. 159:77. 13. Levy-Nissenbaum, E. et al. (2003) Nat. Genet. 34:151.