CORO1A (Coronin-1A, Coronin-like Protein A, Clipin-A, Coronin-like Protein p57, Tryptophan Aspartate-containing Coat Protein, TACO, CORO1)

Coronin 1 was first isolated from Dictyostelium, but similar proteins have been identified in many species and individual cell types. The coronin-like protein in yeast promotes actin polymerization and also interacts with microtubules. Dictyostelium mutants lacking coronin are impaired in cytokinesis and all actin-mediated processes. Analysis of coronin-GFP fusions and knockout mutants shows that coronin participates in the remodelling of the cortical actin cytoskeleton that is responsible for phagocytosis and macropinocytosis. Likewise, in mammalian neutrophils, a coronin-like protein is also associated with the phagocytic apparatus. Coronin 1 binds actin and is found in noninfected macrophages, localised to the cortical microtubule network; in mycobacteria-infected macrophages, progressively relocalized and retained around the mycobacterial phagosomes.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: >1:16,000\nWestern Blot: 0.25-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat or Molt-4\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7901-36L

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ferrari G, Langen H, Naito M, Pieters J.A coat protein on phagosomes involved in the intracellular survival of mycobacteria.Cell. 1999 May 14;97(4):435-47. PMID: 10338208 2. Honor

Additional Info

Recognizes human CORO1A. Species sequence homology: mouse and rat.