Cortactin (p80/85 src substrate, Src substrate cortactin, Amplaxin, CTTN, EMS1, Mammary tumor and squamous cell carcinoma associated, Oncogene EMS1)

Cortactin is an 80/85kD cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks.\n\nCellular Localization: Cytoplasmic\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7901-83F

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Schuuring E et al. Identification and cloning of two overexpressed genes, U21B31/PRAD1 and EMS1, within the amplified chromosome 11q13 region in human carcinomas. Oncogene 7:355-61 (1992). <PUBMED:1532244>

Additional Info

Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse, Rat due to sequence homology.

Alternative Names

p80/85 src substrate, Src substrate cortactin, Amplaxin, \nCTTN, EMS1, Mammary tumor and squamous cell carcinoma associated, Oncogene EMS1