Validated for a wide range of fecal, serum and plasma samples
Simple, stable kit stored at 4Degree C
Strong signals over a wide range
The Corticosterone Enzyme Immunoassay kit measures corticosterone present in extracted fecal samples and in serum and plasma samples. The kit has been developed to measure extracted fecal samples and has been validated with nine species of animal samples. A corticosterone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody. A corticosterone-peroxidase conjugate is added to the wells. binding reaction is initiated by the addition of a polyclonal antibody to corticosterone. After an hour incubation the plate is washed and substrate is added. substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is read at 450 nm wavelength. The concentration of the corticosterone in the sample is calculated using software available with most plate readers.
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