Corticotropin Releasing Hormone Binding Protein (CRHBP)

Human CRHBP is a 37kD secreted glycoprotein that likely serves to regulate CRH availability to the adrenal. In human, CRHBP complexes with CRH to form a circulating 41kD complex. In rodent and horse, this complex is restricted to the CNS. Human CRHBP is synthesized as a 322 amino acid (aa) precursor that contains a 24 aa signal sequence and a 298 aa mature region. The mature region contains one potential CUB domain and five intrachain disulfide bonds. Human mature CRHBP shares 87% and 84% sequence identity with mouse and equine CRHBP, respectively.\n\nHybridoma: \nProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Cortieotrophin-Releasing Hormone Binding Protein (rhCRHBP; aa 25-322: Accession # CAA51052).\n\nApplications: \nWestern Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human CRHBP. Using a colorimetric detection system, the detection limit for rhCRHBP is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human CRHBP. The detection limit for rhCRHBP is approximately 15 ng/well.\nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7903-99A

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Selected for its ability to detect human CRHBP in direct ELISAs and Western Blots. In these applications, this antibody shows no crossreactivity with rmCRHBP.