The Cortisol Immunoassay kit is designed to quantitatively measure cortisol present in dried fecal extracts and in saliva, urine, serum, plasma and tissue culture media samples. This kit measures total cortisol in serum and plasma and free cortisol in urine. A cortisol standard is provided to generate a standard curve for the assay.
Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After a 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is read at 450 nm wavelength. The concentration of the cortisol in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers.
Cortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the
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