The B-Bridge Cortisone Chemiluminescent Immunoassay kit is designed to quantitatively measure Cortisone present in samples. A cortisone standard is provided to generate a standard curve for the assay. After a two hour incubation the plate is washed and the chemiluminescent substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce light. The generated light is detected in a multilabel microtiter plate reader.
Cortisone and cortisol concentrations vary due to the activity of two 11 Beta-hydroxysteroid dehydrogenases (11-HSD). While most tissues have the ability to express either enzyme, 11 Beta-HSD1 is found primarily in the liver where it converts cortisone to cortisol while 11 Beta-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. 11 Beta-HSD2 deactivates cortisol to cortisone, prohibiting receptor activation. This glucocorticoid
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