The Cortisone Immunoassay kit is designed to quantitatively measure cortisone present in se-rum, urine, saliva, dried fecal extracts and tissue culture media samples. A cortisone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a white microtiter plate coated with an antibody to capture rabbit IgG. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a 2 hour incubation, the plate is washed and the supplied chemiluminescent substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate and the intensity of the generated light emission is detected in a microtiter plate reader. The concentration of the cortisone in the sample is cal-culated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
Serum, urine, saliva, dried fecal extracts and tissue culture media samples.
Quick Search
Products 
