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COT, Recombinant, Human, GST-Tag (Cancer Osaka Thyroid Oncogene, c-COT, EST, ESTF, Mitogen-activated Protein Kinase Kinase Kinase 8, MAP3K8, MEKK8, Proto-oncogene c-Cot, Serine/threonine-protein Kinase Cot, Tumor Progression Locus 2, TPL2, TPL-2)

Cat no: C7904-43

COT, Recombinant, Human, GST-Tag (Cancer Osaka Thyroid Oncogene, c-COT, EST, ESTF, Mitogen-activated Protein Kinase Kinase Kinase 8, MAP3K8, MEKK8, Proto-oncogene c-Cot, Serine/threonine-protein Kinase Cot, Tumor Progression Locus 2, TPL2, TPL-2)

Human Cot/Tpl2 (GenBank Accession No. NM_005204), (aa30-397), with N-terminal GST-HIS6-tag, expressed in a Baculovirus infected Sf9 cell expression system.\n\nSpecific Activity: \n~40pmol/min/ug \n\nAssay Conditions: \n100ul of 240nm biotinylated MEK1 peptide was coated onto a neutravidin plate for 1 hour at 37C. After overnight blocking with TBS superblock, the reaction was done with 10uM ATP for 60 minutes at RT, and phospho-MEK1 was detected using an anti-phospho-MEK1 antibody. Specific activity was determined relatively by comparing with a standard kinase under the same condition.\n\nApplications: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.

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SPECIFICATIONS

Catalog Number

C7904-43

Size

10ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 3mM DTT., 50% glycerol

Purity

~75%

References

1. Stafford, M.J. et al., FEBS Lett. 580, 4010-4014 (2006) 2. Babu, G. et al., J. Biol. Chem. 281, 14041-14047 (2006)

Alternative Names

EC=2.7.11.25

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