COX 1 (Cyclooxygenase-1, PGHS-1, Prostaglandin Endoperoxide Synthase-1, PHS 1, Ovarian Cancer Marker)

The prostanoid family includes PGD2, PGE2, PGF2alpha, PGI2, thromboxane A2 and prostaglandins. The prostaglandins (PGs) are implicated in various physiological and pathophysiological events, including male fertility, menstruation, ovulation, pregnancy, implantation and inflamatory and neoplastic diseases. The biosynthesis of PGs and some other prostanoids, is catalyzed in a rate limiting step by PG-H synthase (also known as cyclooxygenase (COX), PG-endoperoxidase synthase (PTGS)) which converts arachiodonic acid to prostaglandin/prostanoid precursor PGH2. Two cyclooxygenase isozymes, COX1 (human, 576aa, 69-72kD; chromosome 9) and COX2 (human, 604aa, 74kD; chromosome 1) have been identified. COX1, a constitutively expressed isoform, produces physiologically relevant prostanoids such as those in stomach and platelets. COX2 isoform is inducible and rapidly upregulated at inflamation sites and forms proinflamatory prostanoids. The overexpression of COX-2 also leads to tumerogenesis. Recently, a third isoform COX3 (canine 633aa; ~65kD in human aorta) has been reported. Two smaller COX1-derived proteins (partial COX1) PCOX1a (canine 414aa, ~53kDa in human aorta) and PCOX1b have also been characterized. The COX3, but not PCOX1a, possesses glycosylation dependent cyclooxygenase activity. The nonsteroidal antiinflammatory drugs (NSAIDs) reduce the formation of prostaglandins by inhibiting the activity of cyclooxygenases (COX1, COX2 and COX3), This ability was associated with inhibition of COX, which converts arachidonic acid to the prostaglandin precursor prostaglandin H2.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution: \nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7904-76C

Size

1ml

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum, Bov, GP, Rabbit, Sheep

Form

Supplied as a liquid, 0.1% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG2b

References

1. DeWitt, D.L., et al., Concentrations of PGH Synthase and PGI2 Synthase in the Endothelium and Smooth Muscle of Bovine Aorta. J. Clin. Invest. 72: 1882 (1983).2. DeWitt, D.L., et al., Orientation of the Active Site and Antigenic Determinants of Prostaglandin Endoperoxide (PGH) Synthase in the Endoplasmic Reticulum. J. Biol. Chem. 256: 10375 (1981).3. Pagels, W.R., et al., Immunochemical Evidence for the Involvement of Prostaglandin Endoperoxide Synthase in Hydroperoxide-Dependent Oxidations by Ram Seminal Vesicles. J. Biol. Chem. 258: 6517 (1983).4. Smith, W.L. and Rollins, T.E., Characteristics of Rabbit Anti-PGH Synthase Antibodies and Use in Immunochemistry. Methods in Enzymology 86: 213 (1982).5. DeWitt, D.L., et al., Monoclonal Antibodies Against PGH Synthase: An Immunoradiometric Assay for Quantitating the Enzyme. Methods in Enzymology 86: 229 (1982).

Additional Info

Recognizes COX 1. Species crossreactivity: sheep, bovine, guinea pig, rabbit and human. Does not react with rat, mouse or canine.