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COX 2, CT (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

COX 2, CT (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Cat no: C7904-80N

Supplier: United States Biological
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Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b. Cellular Localization: Membrane associated. Microsomal membrane. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7904-80N
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 50ul
Form: 0.02M K Phosphate, 0.15M NaCl, pH7.2
P type: Pab
Isotype: IgG
Purity: Whole antisera
Alternative names: COX 2, COX2, Cyclooxygenase 2, Cyclooxygenase, Cyclooxygenase2, PGG/HS, PGH synthase 2, PGHS 2, PGHS2, PHS 2, PHS II, PHS2, PTGS2, Prostaglandin G/H synthase 2 precursor, Prostaglandin G/H synthase and cyclooxygenase, Prostaglandin G/H synthase, Prostaglandin H2 synthase 2, Prostaglandin endoperoxide synthase 2, TIS10, hCox 2
References: This product has been used in:Tong M & Tai HH Induction of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase expression by androgens in human prostate cancer cells. Biochem Biophys Res Commun 276:77-81 (2000). Guan Z et al. Interleukin-1beta-induced cyclooxygenase-2 expression requires activation of both c-Jun NH2-terminal kinase and p38 MAPK signal pathways in rat renal mesangial cells. J Biol Chem 273:28670-6 (1998).
Additional info: The antibody does not cross react with Cox-1. Species Crossreactivity: Crossreacts with Human, Rat and Mouse.

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