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COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Cat no: C7904-82F

Supplier: United States Biological
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Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions.10 COX-2 is a 72kDa protein which has been cloned from a variety of species including human, mouse, rat, and sheep. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml ( 1:1000) Immunohistochemistry: 1:50-1:100 Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7904-82F
Reactivities: Human, Non-Human Primate, Sheep
Hosts: Mouse
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a lyophilized powder.Reconstitution: Reconstitute with 100ul sterile, ddH2O or sterile 40-50% glycerol.
P type: Mab
Isotype: IgG1
Purity: Purified.
Additional info: Recognizes human COX-2. Species Crossreactivity: monkey and sheep. Does not crossreact with mouse, rat or rabbit COX-2 or COX-1.

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