COX 2, Human, Control Peptide (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2)

Control Peptide for C7904-83 (affinity purified) or C7904-80H (serum).\n\nThe prostanoid family includes PGD2, PGE2, PGF2alpha, PGI2, thromboxane A2 and prostaglandins. The prostaglandins (PGs) are implicated in various physiological and pathophysiological events, including male fertility, menstruation, ovulation, pregnancy, implantation and inflamatory and neoplastic diseases. The biosynthesis of PGs and some other prostanoids is catalyzed in a rate limiting step by PG-H synthase (also known as cyclooxygenase (COX), PG-endoperoxidase synthase (PTGS)) which converts arachiodonic acid to prostaglandin/prostanoid precursor PGH2. Two cyclooxygenase isozymes, COX1 (human, 576aa, 69-72kD; chromosome 9) and COX2 (human, 604aa, 74kD; chromosome 1) have been identified. COX1 is a constitutively expressed isoform. it produces physiologically relevant prostanoids such as those in stomach and platelets. COX2 isoform is inducible. it is rapidly upregulated at inflamation sites. It forms proinflamatory prostanoids. The overexpression of COX2 also leads to tumerogenesis. Recently, a third isoform COX3 (canine 633aa; ~65kD in human aorta) has been reported. Two smaller COX1-derived proteins (partial COX1) PCOX1a (canine 414aa, ~53kD in human aorta) and PCOX1b have also been characterized. The COX3, but not PCOX1a, possesses glycosylation dependent cyclooxygenase activity. The nonsteroidal anti-inflammatory drugs (NSAIDs) reduce the formation of prostaglandins by inhibiting the activity of cyclooxygenases (COX1, COX2 and COX3). This ability was associated with inhibition of COX, which converts arachidonic acid to the prostaglandin precursor prostaglandin H2.\n\nCOX2 is a 74kD protein having 60% homology with COX1. It is expressed by extracellular stimuli such as tumor promoters, pro-inflamatory cytokines, mitogensand oncogenes in different cells. The control of COX2 gene expression is regulated at the level of transcriptional and post-transcriptional mechanisms.\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low MW <3kD. Other applications not tested.\n\nRecommended Dilution: \nELISA and Antibody Blocking:\n5-10ug per 1ug C7904-83 (affinity purified antibody) or 1ul C7904-80H (serum). Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7904-81

Size

50ug

Applications

ELISA

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.5

Purity

Highly purified

References

1. Hla, T., Neilson, K., PNAS USA 89: 7384-7388 (1992). 2. Jones, D.A., JBC 268: 9049-9054 (1993). 3. Yamagata, K., et al., Neuron 11: 371-386 (1993). 4. Ryseck, R.P., et al., Cell Growth Differ. 3(7): 443 (1992).

Additional Info

Synthetic peptide (17aa) near the C-terminus of Human COX2. Species Sequence Homology: Ovine, murine and rat: 100%. Sequence homology: 3T3 cells, BMMC, L-929, PC-12, C127, CEF-147.\n