COX 2 (PTGS2, Prostaglandin-endoperoxide Synthase 2, Prostaglandin G/H Synthase and Cyclooxygenase, COX2, COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, Prostaglandin G/H Synthase and Cyclooxygenase)

Cyclooxygenase (COX) also known as PTGS catalyzes the conversion of arachidonic acid to prostaglandin H2 (PG), the common biosynthetic precursor to prostaglandins and thromboxane. The enzyme COX exists in two isoforms, encoded by distinct genes. COX-1 is constitutively expressed in most tissues, while COX-2 is primarily an inducible enzyme (constitutively expressed in brain and kidney), whose expression is activated in a variety of cells in response to cytokines, mitogens and endotoxins. COX2 exhibits 86%-89% aa sequence identity with mouse, rat, sheep, bovine, horse and rabbit PTGS2 proteins, respectively. It exists as homodimer with a molecular mass of approximately 70kD per monomer and each subunit composed of three distinct structural domains: a short N-terminal epidermal growth factor (EGF) domain; an alpha-helical membrane-binding motif; and a C-terminal catalytic domain. It acts as a major mediator of inflammation and plays a role in prostanoid signaling. It is expressed throughout the forebrain in discrete populations of neurons and is enriched in the cortex and hippocampus. The expression of Cox-2 gene is deregulated in epithelial tumors. The gene is mapped to human chromosome 1q25.2-q25.3.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nELISA: >1:32,000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nH460\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7904-82G

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ballif BA, Mincek NV, Barratt JT, Wilson ML, Simmons DL. Interaction of cyclooxygenases with an apoptosis- and autoimmunity associated protein.Proc Natl Acad Sci USA. 1996 May 28;93(11):5544-9. PMID: 8643612.

Additional Info

Recognizes human COX2. Species sequence homology: bovine, equine, porcine, ovine.