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COX5B, Recombinant, Human, aa32-129, His-Tag (Cytochrome C Oxidase Subunit 5B Mitochondrial Precursor, Cytochrome C Oxidase Subunit 5B, Cytochrome C Oxidase Polypeptide VB Mitochondrial, Cytochrome C Oxidase Subunit Vb)

Cat no: 137030

COX5B, Recombinant, Human, aa32-129, His-Tag (Cytochrome C Oxidase Subunit 5B Mitochondrial Precursor, Cytochrome C Oxidase Subunit 5B, Cytochrome C Oxidase Polypeptide VB Mitochondrial, Cytochrome C Oxidase Subunit Vb)

Cytochrome c oxidase subunit 5B, also known as COX5B, is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels within the cell are high, transcription of COX5A (the aerobic isoform) is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5B (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme. \n\nSource: \nRecombinant protein corresponding to human COX5B, fused to His-tag at N- terminal expressed in E.coli.\n\nMolecular Weight: \n~3kD (121aa) (MALDI-TOF)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSASGGGVP TDEEQATGLE REIMLAAKKG LDPYNVLAPK GASGTREDPN LVPSISNKRI VGCICEEDNT SVVWFWLHKG EAQRCPRCGA HYKLVPQQLA H\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137030

Size

20ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.1M NaCl, 20% glycerol, 1mM DTT.

Purity

~95% (SDS-PAGE)

References

1. Bueke P V., et al. (1998) J Exp Biol. 201:1163-1175.

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