CpG-C DNA, Human, Mouse, BioGenomics(TM)

ODN 2395 is a prototype of the class of CpG-C oligodeoxynucleotides (ODN) and is particularly effective at activating B- and NK cells and inducing IFN-alpha production in vitro of human peripheral blood mononuclear cells (PBMC). The vertebrate immune system has evolved innate immune defense pattern recognition receptors (PRRs) that detect unmethylated cytosine-phosphate-guanine (CpG) motifs within bacterial DNA. Cellular activation by CpG motifs occurs via the Toll signal pathway. The Toll-like receptor-9 (TLR9, CD289) appears to be a major component of the CpG-DNA receptor, acting by direct binding to CpG-DNA, which triggers the induction of cell signaling pathways including the mitogen activated protein kinase (MAPKs) and NFkB, leading to stimulation of various cells of the immune system. The human TLR9 is expressed in B cells and plasmacytoid dendritic cells (PDC). Mice also express TLR9 in the myeloid compartment. Optimal sequences for activating TLR9 vary among species. Synthetic ODN contain CpG-DNA motifs mimicking the immunostimulatory effects of bacterial DNA and can, therefore, be used as immunoprotective agents, vaccine adjuvants and anti-allergic agents. CpG ODN also affects immune tolerance and autoimmunity. Different classes of CpG ODN are characterized each with distinct effects on the immune response: CpG-A (

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SPECIFICATIONS

Catalog Number

C7905-84

Size

200nmol

Form

Supplied as a lyophilized powder. Endotoxin levels are undetectable. Reconstitute with sterile ddH2O.

Purity

Purified

References

1. Krieg, A; CpG motifs in bacterial DNA and their immune effects. Annu Rev Immunol 2002, 20: 709 \n2. Vollmer, J et al; Characterization of three CpG oligodeoxynucleotide classes with distinct \nimmunostimulatory activities. Eur J Immunol 2004, 34: 251 3. Jurk, M et al; C-class CpG ODN: sequence requirements and characterization of immunostimulatory activities on mRNA level. Immunobiology 2004, 209: 141 4. Sivori, S et al; Comparison of different CpG oligodeoxynucleotide classes for their capability to \nstimulate human NK cells. Eur J Immunol 2006, 36: 1-7 \n