CRABP I (Cellular Retinoic Acid Binding Protein I)

Retinoic acid (RA), a metabolite of vitamin A, plays an important role in growth and differentiation by modulation of expression of certain genes. RA functions through its interaction with the nuclear protein, retinoic acid receptor (RAR) and the 9-cis RA with retinoid X receptor (RXR). The Cellular Retinoic Acid Binding Proteins (CRABP) bind RA with a high affinity and belong to the family of fatty acid binding proteins. Two forms of CRABP have been characterized, CRABP 1 and CRABP 2 which share ~75% sequence conservation. The main role of CRABP 1 may be to protect retinoids from non-specific dehydrogenases and direct them to specific enzymes. The precise role of CRABP 2 is still poorly understood but it seems to play a role in the interaction of ligands (RA, 9-cis RA) with nuclear receptors (RAR/RXR).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7906-70B

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Bov, Ch/Bird, Fel, Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2b

References

Kelley MW et al. Retinoic acid promotes differentiation of photoreceptors in vitro. Development 120:2091-102 (1994). <PUBMED:7925013>Kelley MW et al. The developing organ of Corti contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture. Development 119:1041-53 (1993). <PUBMED:8306874>Gaur VP et al. Localization of cellular retinoic acid-binding protein to amacrine cells of rat retina. Exp Eye Res 50:505-11 (1990). <PUBMED:2164945>Milam AH et al. Immunolocalization of cellular retinoic acid binding protein to Muller cells and/or a subpopulation of GABA-positive amacrine cells in retinas of different species. J Comp Neurol 296:123-9 (1990). <PUBMED:2162866>De Leeuw AM et al. Immunolocalization of cellular retinol-, retinaldehyde- and retinoic acid-binding proteins in rat retina during pre- and postnatal development. J Neurocytol 19:253-64 (1990). <PUBMED:2162910>

Additional Info

Detects cellular Retinoic Acid Binding Protein 1 (CRABP 1). This antibody does not crossreact with Cellular Retinol Binding Protein (CRBP), performic acid oxidized CRBP, interphotoreceptor retinoid binding protein or retinol binding protein. Species Crossreactivity: Crossreacts with Human, Rabbit, Rat, bovine, Cat, Chicken, Primate and Reptiles.