CRACC, also known as CSI (CD2 subset I), is a type I transmembrane protein belonging to
the CD2 subset of the Ig superfamily. CRACC is expressed on most NK cells and subsets of
CD8 + cells, CD4 + cells and B cells. CRACC may play a role in the activation and effector
function of T cells and NK cells.
Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 2ug/ml. Lysates were prepared from mouse thymus extract in reducing sample
buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. Use of this antibody under non-reducing conditions is not recommended. The blot was developed with 2ug/ml antibody and chemiluminescent detection substrate.
ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse CRACC. The detection limit for rmCRACC is approximately 1ng/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.