CRACC, also known as CSI (CD2 subset I), is a type I transmembrane protein belonging to the CD2 subset of the Ig superfamily. CRACC is expressed on most NK cells and subsets of CD8 + cells, CD4 + cells and B cells. CRACC may play a role in the activation and effector function of T cells and NK cells.
Applications:
Suitable for Direct ELISA and Flow Cytometry. Other Applications not tested.
Recommended Dilutions:
Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in a total reaction volume <200ul.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.