Cre (Cyclization Recombinase, Recombinase Cre)

The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression. As an example, mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells. IMG-5720 recognizes the recombinase protein, Cre. Cre is a 343aa protein, GenBank no. AAQ14086.1.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nImmunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000\nImmunoprecipitation: 1:50-1:200\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7909-03B

Size

50ul

Applications

IHC, IP, WB

Hosts

Rabbit

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Branda CS and Dymecki SM, 2004. Talking about a revolution: the impact of site-specific recombinases on genetic analysis in mice. Developmental Cell. 6:7-28. 2. Gilbertson L. Cre-lox recombination: Cre-ative tools for plant biotechnology. TRENDS in Biotechnology 21:550-555. 3. Ghosh K and GD Van Duyne. 2002. Cre-loxP biochemistry. Methods 28:374-383.

Additional Info

Recognizes Cre.