Cre-recombinase is a 38kD DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family. C7920-01 is an important tool for the identification of Cre recombinase in different protein expression systems.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.1-1.0ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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