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Cre Recombinase

Cat no: C7920-01

Cre Recombinase

Cre-recombinase is a 38kD DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family. C7920-01 is an important tool for the identification of Cre recombinase in different protein expression systems.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.05-0.2ug/ml\nWestern Blot: 0.1-1.0ug/ml \nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nSpleen cells were fused with SP2/0-Ag14 mouse myeloma cells\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7920-01

Size

40ug

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes Cre Recombinase.

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