CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)

The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500-1:2000. Detects bands of 46 and 43kD corresponding to phosphorylated CREB.\nImmunohistochemistry (FFPE): 20ug/ml\nELISA: 1:1000-1:6000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7915-05C

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Moreno, C.S., et al . (1999) CREB regulates MHC class II expression in a CIITA dependent manner. Immunity 10: 143-151.. (2000) MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells. FEBS Lett, 482(1-2): 44-8.2. Lee CQ, Yun YD, Hoeffler JP, Habener JF. (1990) EMBO J. 9(13):4455-4465. 3. Dwarki VJ, Montminy M, and Verma IM. (1990)EMBO J., 9(1):225-232 3.Kwok RP, Laurance ME, Lundblad JR, Goldman PS, Shih H, Connor LM, Marriott SJ, Goodman RH. (1996) Control of cAMP-regulated enhancers by the viral transactivator Tax through CREB and the co-activator CBP. Nature 380:642-646. 5. Barton K, Muthusamy N, Chanyangam M, Fischer C, Clendenin C, Leiden JM. (1996). Nature 379:81-85.Kim J., Lu J. and Quinn PG., (2000) Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation. Proc Natl Acad Sci U S A 97(21):11292-11296.

Additional Info

Recognizes phosphorylated human CREB at pS133. Species Crossreactivity: mouse and rat. Minimal reactivity with non-phosphorylated CREB by Western Blot and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide. Assayed against a variety of tisues including fibroblasts and B-cell lysates.