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CREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein)

CREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein)

Cat no: C7915-05G

Supplier: United States Biological
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CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physi-ological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Chromatin IP: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7915-05G
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide and 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
References: 1. Lonze, B.E. et al. (2002) Neuron 34, 371-85, 2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12, 3. Redmond, L. et al. (2002) Neuron 34, 999-1010, 4. Dash, P.K. et al. (1990) Nature 345, 718-21, 5. Yin, J.C. et al. (1994) Cell 79, 49-58, 6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8, 7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55, 8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107, 9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.
Additional info: Recongnizes endogenous levels of human CREB only when phosphorylated at Ser133. Species Crossreactivity: mouse, rat.

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