CREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein)

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. \nCREB is able to mediate signals from numerous physi-ological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting \nneuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9). \n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nChromatin IP: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C7915-05G

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide and 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85, 2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12, 3. Redmond, L. et al. (2002) Neuron 34, 999-1010, 4. Dash, P.K. et al. (1990) Nature 345, 718-21, 5. Yin, J.C. et al. (1994) Cell 79, 49-58, 6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8, 7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55, 8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107, 9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

Additional Info

Recongnizes endogenous levels of human CREB only when phosphorylated at Ser133. Species Crossreactivity: mouse, rat.