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CREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein)

CREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein)

Cat no: C7915-11A

Supplier: United States Biological
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Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1,000 to 1:4,000 Detects phospho-CREB (Ser133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells. Lysates from untreated (Lanes 1 and 3) and forskolin treated (Lanes 2 and 4) PC-12 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (Ser133) (1:4,000 dilution, Lanes 1 and 2) or anti-CREB (1:2,000 dilution, Lanes 3 and 4). Proteins were visualized using a goat antimouse (Lanes 1and 2) or goat antirabbit (Lanes 3 and 4) secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7915-11A
Reactivities: Mouse, Rat
Hosts: Mouse
Applications: Western Blot
Size: 200ug
Form: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein G affinity chromatography.
References: Ginty, D.D., et al., Science 260: 238-241, 1993.
Additional info: Recognizes phospho-CREB (Ser133), Mr 43kD. Species Crossreactivity: Rat and mouse. Broad species crossreactivity expected, based on sequence homology.

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