CREB is a transcription factor which binds to cyclic AMP response elements (CRE's), and is induced by phosphoyrlation on the part of PKA, CaM K IV, and other protein kinases. Activated CREB will dimerize, bind to DNA, and recruit histone acetyl transferases.
Applications:
Suitable for use in ELISA, Immunohistochemistry, and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot (transfected lysate): 1-5ug/ml detects a band at ~37kD using CREB1 transfected 293T lysate
Immunohistochemistry (paraffin): 1ug/ml using human kidney tissue
ELISA: 1-5ug/ml using recombinant protein. The detection limit for recombinant GST tagged CREB1 is ~1ng/ml
Optimal dilutions to be determined by the researcher.
AA Sequence:
MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQISTIAESE
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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