CREBZF (CREB/ATF bZIP Transcription Factor, Host Cell Factor-binding Transcription Factor Zhangfei, HCF-binding Transcription Factor Zhangfei, ZF, FLJ94018)

ZHANGFEI, also known as ZF, is a basic region-leucine zipper transcriptional factor that has been implicated in herpes virus infection cycle and related cellular processes. ZHANGFEI protein has a negatively charged active N-terminus, a leucine zipper of six heptad leucine repeats separated by a conserved 6aa spacer, a basic domain and a bZIP region. ZHANGFEI strongly activates transcription and suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1 dependent manner. It also suppresses the HCFC1 dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunocytochemistry: 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562 Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146808

Size

100ug

Applications

ELISA, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hogan MR, Cockram GP and Lu R. Cooperative interaction of Zhangfei and ATF4 in transactivation of the cyclic AMP response element. FEBS Lett. 2006; 580:58-62. 2. Cockram GP, Hogan MR, Burnett HF, et al. Identification and characterization of the DNA-binding properties of a Zhangfei homologue in Japanese pufferfish, Takifugu rubripes. Biochem. Biophys. Res. Commun. 2005; 339:1238-45. 3. Lu R and Misra V. Zhangfei: a second cellular protein interacts with herpes simplex virus accessory factor HCF in a manner similar to Luman and VP16. Nucleic Acids Res. 2000; 28:2446-54. 4. Misra V, Rapin N, Akhova O, et al. 2005. Zhangfei is a potent and specific inhibitor of the host cell factor-binding transcription factor Luman. J. Biol. Chem. 2005; 280:15257-66.

Additional Info

Recognizes human ZHANGFEI. Species Crossreactivity: mouse.