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CRELD1, Recombinant, Human, aa30-362, His-tag (Cysteine-rich with EGF-like Domain Protein 1, AVSD2, CIRRIN)

Cat no: 145785

CRELD1, Recombinant, Human, aa30-362, His-tag (Cysteine-rich with EGF-like Domain Protein 1, AVSD2, CIRRIN)

Cysteine-rich with EGF-like domain protein 1 (CRELD1) is an ~50kD transmembrane glycoprotein that contains a 333 amino acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15aa (1). Alternative splicing of human CRELD1 generates an additional isoform with a substitution that encompasses the transmembrane segments and the C-terminal ECD (1). CRELD1 is widely expressed during development and in the adult (1). It interacts with Reticulon 3/RTN3 and inhibits the pro-apoptotic activity of RTN3 (2). CRELD1 also associates with Adrenergic alpha1 receptors in the prostate and limits their expression (3). Missense mutations of human CRELD1 are associated with susceptibility to atrioventricular septal defects in the heart (4). \n\nSource:\nRecombinant protein corresponding to aa30-362 from human CRELD1, fused to his-tag at C-terminal, NS0-derived.\n\nMolecular Weight: \n~50-55kD\n\nEndotoxin:\n<0.10EU/1ug (LAL method).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145785

Size

50ug

Form

Supplied as a lyophilized powder in PBS. Reconstitute in 250ug/ml PBS.

Purity

~95% (SDS-PAGE)

References

1. Rupp, P.A. et al. (2002) Gene 293:47. 2. Xiang, R. and S. Zhao (2009) Mol. Cell. Biochem. 331:225. 3. Nishimune, A. et al. (2010) J. Pharmacol. Sci. 113:169. 4. Robinson, S.W. et al. (2003) Am. J. Hum. Genet. 72:1047.

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