CRIPTO, NT (Teratocarcinoma-derived Growth Factor 1, Epidermal Growth Factor-like Cripto Protein CR1, Cripto-1 Growth Factor, CRGF, TDGF1) (APC)

The human cDNA for CRIPTO, isolated from a teratocarcinoma cell line, encodes a protein of 188aa. The central section has structural similarities with the human transforming growth factor alpha and epidermal growth factor (EGF). CRIPTO transcripts are detected only in undifferentiated cells and disappear after cell differentiation induced by retinoic acid treatment. The EGF-CFC (Cripto, Frl1, and Cryptic) gene family has an important role in vertebrate development. Cripto is required for germ-layer formation and the correct positioning of the anterior-posterior axis in mic; Cryptic (CFC1) is necessary for assignment of the left-right axis. Prior to gastrulation, Cripto expression is initially symmetric and uniform in the epiblast, and then becomes asymmetric in a proximal-distal gradient. Cripto gene when rendered inactive is fatal in the murine model. Cripto and Cryptic are essential cofactors for Nodal family proteins, which transduce signals for mesoderm development. Cryptic protein is required for appropriate symmetry development in humans. TDGF1, like CFC1, is an EGF-CFC family member and required coreceptor in NODAL signaling, a developmental program implicated in midline, forebrain, and left-right axis development in model organisms. A mutation in the conserved CFC domain of the TDGF1 gene has been identified in a patient with midline anomalies of the forebrain. The mutant protein was inactive in a zebrafish rescue assay.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:1000\nImmunohistochemistry: 1:10-1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7922-10K-APC

Size

200ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Parisi, S., et al., J. Cell Biol. 163(2):303-314 (2003). 2. Gray, P.C., et al., Proc. Natl. Acad. Sci. USA 100(9):5193-5198 (2003). 3. Yan, Y.T., et al., Mol. Cell. Biol. 22(13):4439-4449 (2002). 4. de la Cruz, J.M., et al., Hum. Genet. 110(5):422-428 (2002). 5. Dono, R., et al., Am. J. Hum. Genet. 49(3):555-565 (1991).

Additional Info

Recognizes human CRIPTO.