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CRISP-3 (Cysteine-rich Secretory Protein 3, Specific Granule Protein of 28kD, SGP28, CRISP3)

CRISP-3 (Cysteine-rich Secretory Protein 3, Specific Granule Protein of 28kD, SGP28, CRISP3)

Cat no: C9018-57

Supplier: United States Biological
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Cysteine-rich secretory protein 3 (CRISP-3; also known as SGP28) is a glycoprotein that belongs to the family of cysteine-rich secretory proteins (CRISPs) which was originally discovered in human neutrophilic granulocytes. CRISP-3 is also widely distibuted in exocrine glands (salivary glands, pancreas and prostate), eosinophilic granulocytes and to a lower level in epididymis, ovary, thymus and colon. The presence of CRISP-3 in neutrophils, eosinophils and in exocrine secretions indicates a role in innate host defense. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:10 Immunohistochemistry: Paraffin Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C9018-57
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Kjeldsen, L et al; SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein. FEBS Lett 1996, 380: 246. 2. Udby, L et al; An ELISA for SGP28/CRISP-3, a cysteine-rich secretory protein in human neutrophils, plasma, and exocrine secretions. J Immunol Methods 2002, 263: 43. 3. Udby, L et al; Identification of human cysteine-rich secretory protein 3 (CRISP-3) as a matrix protein in a subset of peroxidase-negative granules of neutrophils and in the granules of eosinophils. J Leukoc Biol 2002, 72: 462.
Additional info: Recognizes both the N-glycosylated and non-glycosylated form of mature human CRISP-3.

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