CRISP1, CT (CRISP1, AEGL1, Cysteine-rich secretory protein 1, AEG-like protein, ARP, Acidic epididymal glycoprotein homolog) (AP)

Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. This protein is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface. Two isoforms are encoded by transcript variants of this gene.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

034229-AP

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Mungall, A.J., et al. Nature 425(6960):805-811(2003)\nEvans, J.P. Hum. Reprod. Update 8(4):297-311(2002)\nCuasnicu, P.S., et al. Arch. Med. Res. 32(6):614-618(2001)\nCohen, D.J., et al. Biol. Reprod. 65(4):1000-1005(2001)\nKirchhoff, C. Rev. Reprod. 3(2):86-95(1998)

Additional Info

Human

Alternative Names

CRISP1, AEGL1, Cysteine-rich secretory protein 1, AEG-like protein, ARP, Acidic epididymal glycoprotein homolog