Crk II, phosphorylated (Tyr221)

CrkII, a cellular homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases (1). The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk (2). The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mamalian CrkII proteins (3). Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase (4), IGF-I receptor (5) and EGF receptor (6). Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecular folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex (3).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nNote: for Western Blots incubate membrane with diluted antibody in 5% BSA, 1X TBS, and 0.1% Tween-20 at 4C with gentle shaking overnight. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7924-50

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1. Zvara, A. et al. 2001, Oncogene 20, 951-961. 2. Kiyokawa, E. et al. 1997, Crit. Rev. Oncog. 8, 329-342. 3. Rosen, S. K. et al. 1995, Nature 374, 477-479. 4. Amoui, M. and Miller, W.T. 2000, Cell. Signal. 12, 637-643. 5. Koval, A. P. et al. 199,. Biochem. J. 330, 923-932. 6. Hashimoto, Y. et al. 1998, J. Biol. Chem. 273, 17186-17191.

Additional Info

Detects endogenous levels of CrkII only when phosphorylated at tyrosine 221 at 42kD. Crossreactivity: Tyr207 phosphorylated CrkL. Does not crossract wth other tyrosine-phosphorylated proteins. Species Crossreactivity: Human and hamster. Species sequence homology: mouse and rat 100%.