CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)

CrkL is a widely-expressed 39kD adaptor protein that belongs to the Crk protein family and is \nabundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL \ninitiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G, \nSOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and \nthe immune response. Furthermore, this adaptor protein plays an essential role in Chronic \nMyelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase.\n \nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot:1ug/ml. Lysates from human K562, A431 and Jurkat cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml mouse anti-CrkL\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7925-03B

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.

P Type

Mab

Purity

Purified by Protein G immunoaffinity chromatography.

Isotype

IgG1

Additional Info

Detects endogenous human, mouse and rat CrkL at 39kD using Western blot.