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CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)

CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)

Cat no: C7925-03B

Supplier: United States Biological
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CrkL is a widely-expressed 39kD adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL initiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and the immune response. Furthermore, this adaptor protein plays an essential role in Chronic Myelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot:1ug/ml. Lysates from human K562, A431 and Jurkat cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml mouse anti-CrkL Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7925-03B
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Western Blot
Size: 100ug
Form: Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G immunoaffinity chromatography.
Additional info: Detects endogenous human, mouse and rat CrkL at 39kD using Western blot.

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