Crry (Complement Regulatory Protein Crry, Antigen 5I2, Complement Component (3b/4b) Receptor 1-like, Cr1) (FITC)

Complement comprises a system of soluble serum proteins that, upon activiation by antibody (classical pathway), sugars (lectin pathway), or bacterial and foreign surfaces (alternative pathway), directly causes cell damage. Complement is constantly activated at low levels in serum, requiring cells to be protect themselves from damage. In human, cells are protected from complement-mediated damage by expression of membrane-bound complement regulatory proteins, which act at serveral points along the cascade. Human cells are protected by decay-accelerating factor (DAF, CD55), membrane cofactor protein (MCP, CD46) and membrane attack complex inhibitor protein 1 (MIN1, CD59). Furthermore the rat analogues of DAF, MCP and CD59 have been characterized. Rats and mice express a unique membrane complement regulator of the activating pathways, called complement receptor type-1 related gene Y (Crry). In rats there are two isoforms of Crry, with six or seven short consensus repeats (SCR), encoding 65-70 and 75-85kD proteins, respectively, which are both transmembrane anchored. Although Crry has the functional properties of both DAF and MCP, it does not replace them. All rat blood cells express Crry whereas approximately 37 and 39% of the rat T cells express respectively DAF and CD59. Studies in rat revealed that despite lacking DAF and CD59, these double-negative rat T cells were not more susceptible to homologous complement lysis than cells that expressed both DAF and CD59. However, blocking of the only other known rat complement regulator, Crry, enhanced lysis only at the double-negative cells. In rats, Crry exerts powerful control in the activation pathways where it acts both as a cofactor and as a decay accelerator to inactivate convertases. Crry has been succesfully expressed and refolded in bacteria. The refolded protein has full-complement regulatory activity in vitro, and has been applied in rat models of arthritis and other inflammatory diseases. This monoclonal antibody can be used to block autoimmune phenomena in rats.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, ELISA, Immunohistochemistry, Functional Studies and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C2387-07M1

Size

100ug

Applications

ELISA, FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.1% BSA. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Flaris, N et al; Characterization of microglia and macrophages in the central nervous system of rats: definition of the differential expression of molecules using standard and novel monoclonal antibodies in normal CNS and in four models of parenchymal reaction. Glia 1993, 7: 34. 2. McGrath, Y et al; Development of adenovirus vectors encoding rat complement regulators for use in therapy in rodent models of inflammatory diseases. J Immunol 1999, 163: 6834. 3. Fraser, D et al; Bacterial expression and membrane targeting of the rat complement regulator Crry: a new model anticomplement therapeutic. Protein Sci 2002, 11: 2512. 4. Hanna, S et al; Rat T cells express neither CD55 nor CD59 and are dependent on Crry for protection from homologous complement. Eur J Immunol 2002, 32: 502.

Additional Info

Recognizes rat complement regulator Crry.