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CRTC2 (CREB regulated transcription coactivator 2)

Cat no: 030117

CRTC2 (CREB regulated transcription coactivator 2)

Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic b-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes b-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling . The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways . In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting.Tissue specificity: Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas .\n\nApplications:\nWestern Bloting: 1:500-1:2000.\nImmunohistochemistry: 1:200-1:1000.\nImmunofluorescence: 1:200-1:1000.\nFlow cytometry: 1:200-1:400.\nELISA: Suggested dilution 1:10000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

030117

Size

100ul

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, NHP

Form

Ascites fluid, 0.03% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Mol Syst Biol. 2007;3:89. \n2. Nature. 2007 Sep 20;449(7160):366-9. \n3. J Biol Chem. 2009 Mar 20;284(12):8033-41.

Additional Info

Human, Monkey

Alternative Names

TORC2; TORC-2; CRTC2

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info
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