Cryo-electron tomography (cryo-ET) is an imaging technique used for high-resolution three-dimensional (3D) reconstructions of biomolecules, primarily for studying cellular structures. In cryo-ET, vitrified samples are imaged in a transmission electron microscope at tilts ranging from approximately -60deg to +60deg. This generates a series of two-dimensional (2D) images that can be combined to reconstruct a 3D image, known as a tomogram.
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