Cryptochrome 2, Mouse, Control Peptide (CRY2)

Circadian rhythm is one of the most fascinating and complex biological phenomena. The circadian clock controls biological activities on daily light-dark cycles in species from cyanobacteria to humans. The circadian clock has three major components: A photoactive pigment (chromophore) for sensing light and transmitting light signals, the circadian clock that oscillates every ~24hrs, and the genes controlled by the circadian clock to bring about the physiological and behavioral changes. Several genes (Drosophila Clock Per, Tim; mammalian Per1, Per2, Per3, MOP3 and MOP4, and BMAL1) have been linked to rhythmicity or circadian behavior of living organisms. However, very little is known about the mammalian photosensory molecules.\n\nMost recently mammalian homolog of the plant blue-light photoreceptors termed cryptochromes have been identified. Mouse CRY1 and CRY2, are 606aa and 569aa protein, respectively. Cry1 and Cry are specifically expressed in ganglion cell and inner nuclear layers of the mouse retina. CRY1 is expressed at high level in the SCN and oscillates in a circadian manner. Like other genes implicated in circadian mechanism, CRY1 and CRY2 are also expressed in most animal tissues. Therefore, mammalian cryptochromes appears to play an important role in entrainment of the circadian clock.\n\nSequence:\nA 22aa peptide sequence within the C-terminus of mouse CRY2.\n\nSpecies Homology:\n75% conserved in rat, 68% in human CRY2. No significant homology is seen with CRY1.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Control peptide, because of its low MW (< 3kD), is not suitable for Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 50-100ng. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7942-60

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.

Purity

Purified

References

1. Spek, V.D., et al., Genomics 37: 177-182 (1996). 2. Kobayashi, K., et al., Nucl. Acid. Res. 26: 5086-5092 (1997). 3. Lee, C., et al., Cell 107: 855-867 (2001). 4. Field, D., et al., Neuron 25: 437 (2000). 5. Kume, K., et al., Cell 98: 193 (1999). 6. Bae, K., et al., Neuron 30: 525 (2000).